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Gene99
Gibson Station
Gene99
Gibson Station
Twix
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Gibson Station
Twix
Lead time (days)
Cost ($/bp)
Size range (bp)
100
5000
Valid GC%
20
80
Forbidden patterns
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Twix
Gene99
Twix
Assembly Parameters
Cost ($)
Duration (days)
Method
Yeast recombination
Gibson Assembly
Type IIS (Golden Gate)
Oligo assembly
Overhang design
Fixed size
Target Tm
Limit Construct Size
Fragments size
100
5000
Solver Parameters
Grain
Auto
Manual
Use A*
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Gene99
Lead time (days)
Cost ($/bp)
Size range (bp)
500
5000
Valid GC%
30
70
Forbidden patterns
Optimization
Cheapest plan
Cheapest with deadline
Fastest under budget
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